Glial cell line‐derived neurotrophic factor–secreting genetically modified human bone marrow‐derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease
Identifieur interne : 000702 ( Main/Exploration ); précédent : 000701; suivant : 000703Glial cell line‐derived neurotrophic factor–secreting genetically modified human bone marrow‐derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease
Auteurs : Aleksandra Glavaski-Joksimovic [États-Unis] ; Tamas Virag [États-Unis] ; Thomas A. Mangatu [États-Unis] ; Michael Mcgrogan [États-Unis] ; Xue Song Wang [États-Unis] ; Martha C. Bohn [États-Unis]Source :
- Journal of Neuroscience Research [ 0360-4012 ] ; 2010-09.
English descriptors
Abstract
Parkinson's disease (PD) is a neurodegenerative disease characterized by progressive degeneration of nigrostriatal dopaminergic (DA) neurons. The therapeutic potential of glial cell line‐derived neurotrophic factor (GDNF), the most potent neurotrophic factor for DA neurons, has been demonstrated in many experimental models of PD. However, chronic delivery of GDNF to DA neurons in the brain remains an unmet challenge. Here, we report the effects of GDNF‐releasing Notch‐induced human bone marrow‐derived mesenchymal stem cells (MSC) grafted into striatum of the 6‐hydroxydopamine (6‐OHDA) progressively lesioned rat model of PD. Human MSC, obtained from bone marrow aspirates of young, healthy adult volunteers, were transiently transfected with the intracellular domain of the Notch1 gene (NICD) to generate SB623 cells. SB623 cells expressing GDNF and/or humanized Renilla green fluorescent protein (hrGFP) following lentiviral transduction or nontransduced cells were stereotaxically placed into rat striatum 1 week after a unilateral partial 6‐OHDA striatal lesion. At 4 weeks, rats that had received GDNF‐transduced SB623 cells had significantly decreased amphetamine‐induced rotation compared with control rats, although this effect was not observed in rats that received GFP‐transduced or nontransduced SB623 cells. At 5 weeks, rejuvenated tyrosine hydroxylase‐immunoreactive (TH‐IR) fibers that appeared to be host DA axons were observed in and around grafts. This effect was more prominent in rats that received GDNF‐secreting cells and was not observed in controls. These observations suggest that human bone‐marrow derived MSC, genetically modified to secrete GDNF, hold potential as an allogeneic or autologous stem cell therapy for PD. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jnr.22435
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glial cell line‐derived neurotrophic factor–secreting genetically modified human bone marrow‐derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease</title><author><name sortKey="Glavaski Oksimovic, Aleksandra" sort="Glavaski Oksimovic, Aleksandra" uniqKey="Glavaski Oksimovic A" first="Aleksandra" last="Glavaski-Joksimovic">Aleksandra Glavaski-Joksimovic</name></author><author><name sortKey="Virag, Tamas" sort="Virag, Tamas" uniqKey="Virag T" first="Tamas" last="Virag">Tamas Virag</name></author><author><name sortKey="Mangatu, Thomas A" sort="Mangatu, Thomas A" uniqKey="Mangatu T" first="Thomas A." last="Mangatu">Thomas A. Mangatu</name></author><author><name sortKey="Mcgrogan, Michael" sort="Mcgrogan, Michael" uniqKey="Mcgrogan M" first="Michael" last="Mcgrogan">Michael Mcgrogan</name></author><author><name sortKey="Wang, Xue Song" sort="Wang, Xue Song" uniqKey="Wang X" first="Xue Song" last="Wang">Xue Song Wang</name></author><author><name sortKey="Bohn, Martha C" sort="Bohn, Martha C" uniqKey="Bohn M" first="Martha C." last="Bohn">Martha C. Bohn</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8F256686CE630A304B9409E1959F45F60EFAC625</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/jnr.22435</idno><idno type="url">https://api.istex.fr/document/8F256686CE630A304B9409E1959F45F60EFAC625/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000F35</idno><idno type="wicri:Area/Main/Curation">000D58</idno><idno type="wicri:Area/Main/Exploration">000702</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Glial cell line‐derived neurotrophic factor–secreting genetically modified human bone marrow‐derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease</title><author><name sortKey="Glavaski Oksimovic, Aleksandra" sort="Glavaski Oksimovic, Aleksandra" uniqKey="Glavaski Oksimovic A" first="Aleksandra" last="Glavaski-Joksimovic">Aleksandra Glavaski-Joksimovic</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Pediatrics, Neurobiology Program, Children's Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Virag, Tamas" sort="Virag, Tamas" uniqKey="Virag T" first="Tamas" last="Virag">Tamas Virag</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Pediatrics, Neurobiology Program, Children's Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Current Address: National Heart, Lung, and Blood Institute, National Institute of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Mangatu, Thomas A" sort="Mangatu, Thomas A" uniqKey="Mangatu T" first="Thomas A." last="Mangatu">Thomas A. Mangatu</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Pediatrics, Neurobiology Program, Children's Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Mcgrogan, Michael" sort="Mcgrogan, Michael" uniqKey="Mcgrogan M" first="Michael" last="Mcgrogan">Michael Mcgrogan</name><affiliation wicri:level="2"><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>SanBio, Inc., Mountain View</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Xue Song" sort="Wang, Xue Song" uniqKey="Wang X" first="Xue Song" last="Wang">Xue Song Wang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Pediatrics, Neurobiology Program, Children's Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Bohn, Martha C" sort="Bohn, Martha C" uniqKey="Bohn M" first="Martha C." last="Bohn">Martha C. Bohn</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Pediatrics, Neurobiology Program, Children's Memorial Research Center, Feinberg School of Medicine, Northwestern University, Chicago</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Neuroscience Research</title><title level="j" type="abbrev">J. Neurosci. Res.</title><idno type="ISSN">0360-4012</idno><idno type="eISSN">1097-4547</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-09">2010-09</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="2669">2669</biblScope><biblScope unit="page" to="2681">2681</biblScope></imprint><idno type="ISSN">0360-4012</idno></series><idno type="istex">8F256686CE630A304B9409E1959F45F60EFAC625</idno><idno type="DOI">10.1002/jnr.22435</idno><idno type="ArticleID">JNR22435</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0360-4012</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Notch1</term><term>dopamine</term><term>gene therapy</term><term>neurotrophic factor</term><term>nigrostriatal system</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Parkinson's disease (PD) is a neurodegenerative disease characterized by progressive degeneration of nigrostriatal dopaminergic (DA) neurons. The therapeutic potential of glial cell line‐derived neurotrophic factor (GDNF), the most potent neurotrophic factor for DA neurons, has been demonstrated in many experimental models of PD. However, chronic delivery of GDNF to DA neurons in the brain remains an unmet challenge. Here, we report the effects of GDNF‐releasing Notch‐induced human bone marrow‐derived mesenchymal stem cells (MSC) grafted into striatum of the 6‐hydroxydopamine (6‐OHDA) progressively lesioned rat model of PD. Human MSC, obtained from bone marrow aspirates of young, healthy adult volunteers, were transiently transfected with the intracellular domain of the Notch1 gene (NICD) to generate SB623 cells. SB623 cells expressing GDNF and/or humanized Renilla green fluorescent protein (hrGFP) following lentiviral transduction or nontransduced cells were stereotaxically placed into rat striatum 1 week after a unilateral partial 6‐OHDA striatal lesion. At 4 weeks, rats that had received GDNF‐transduced SB623 cells had significantly decreased amphetamine‐induced rotation compared with control rats, although this effect was not observed in rats that received GFP‐transduced or nontransduced SB623 cells. At 5 weeks, rejuvenated tyrosine hydroxylase‐immunoreactive (TH‐IR) fibers that appeared to be host DA axons were observed in and around grafts. This effect was more prominent in rats that received GDNF‐secreting cells and was not observed in controls. These observations suggest that human bone‐marrow derived MSC, genetically modified to secrete GDNF, hold potential as an allogeneic or autologous stem cell therapy for PD. © 2010 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Illinois</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Illinois"><name sortKey="Glavaski Oksimovic, Aleksandra" sort="Glavaski Oksimovic, Aleksandra" uniqKey="Glavaski Oksimovic A" first="Aleksandra" last="Glavaski-Joksimovic">Aleksandra Glavaski-Joksimovic</name></region><name sortKey="Bohn, Martha C" sort="Bohn, Martha C" uniqKey="Bohn M" first="Martha C." last="Bohn">Martha C. Bohn</name><name sortKey="Mangatu, Thomas A" sort="Mangatu, Thomas A" uniqKey="Mangatu T" first="Thomas A." last="Mangatu">Thomas A. Mangatu</name><name sortKey="Mcgrogan, Michael" sort="Mcgrogan, Michael" uniqKey="Mcgrogan M" first="Michael" last="Mcgrogan">Michael Mcgrogan</name><name sortKey="Virag, Tamas" sort="Virag, Tamas" uniqKey="Virag T" first="Tamas" last="Virag">Tamas Virag</name><name sortKey="Virag, Tamas" sort="Virag, Tamas" uniqKey="Virag T" first="Tamas" last="Virag">Tamas Virag</name><name sortKey="Wang, Xue Song" sort="Wang, Xue Song" uniqKey="Wang X" first="Xue Song" last="Wang">Xue Song Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000702 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000702 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:8F256686CE630A304B9409E1959F45F60EFAC625
|texte= Glial cell line‐derived neurotrophic factor–secreting genetically modified human bone marrow‐derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease
}}
| This area was generated with Dilib version V0.6.23. |  |